全文获取类型
收费全文 | 2180篇 |
免费 | 136篇 |
专业分类
林业 | 175篇 |
农学 | 107篇 |
基础科学 | 12篇 |
518篇 | |
综合类 | 112篇 |
农作物 | 110篇 |
水产渔业 | 349篇 |
畜牧兽医 | 747篇 |
园艺 | 52篇 |
植物保护 | 134篇 |
出版年
2023年 | 31篇 |
2022年 | 36篇 |
2021年 | 104篇 |
2020年 | 84篇 |
2019年 | 112篇 |
2018年 | 87篇 |
2017年 | 91篇 |
2016年 | 81篇 |
2015年 | 63篇 |
2014年 | 99篇 |
2013年 | 129篇 |
2012年 | 176篇 |
2011年 | 191篇 |
2010年 | 102篇 |
2009年 | 115篇 |
2008年 | 122篇 |
2007年 | 121篇 |
2006年 | 107篇 |
2005年 | 99篇 |
2004年 | 93篇 |
2003年 | 91篇 |
2002年 | 62篇 |
2001年 | 15篇 |
2000年 | 14篇 |
1999年 | 8篇 |
1998年 | 10篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1995年 | 8篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 5篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 4篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1968年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有2316条查询结果,搜索用时 735 毫秒
71.
72.
Carlos A Rodas Rubén Serna Maria D Bolaños Ginna M Granados Michael J Wingfield 《Southern Forests》2015,77(3):165-171
Adelgids (Hemiptera) in the genus Pineus have been reported as introduced insect pests causing serious losses to Pinus plantations worldwide. In 2008, Pineus boerneri was recorded for the first time in Colombia, with infestations noted on Pinus kesiya, P. tecunumanii, P. maximinoi and P. oocarpa. The lack of information on this insect in Colombia prompted investigations of its life cycle and infestation levels as well as host susceptibility of the main Pinus species planted in Colombia. In addition, the possibility of using a Ceraeochrysa species, an already established predator of adelgids in Colombia, for biological control was considered. Results showed that Pineus boerneri in Colombia has an anholocyclic life cycle comprised of four instars with a complete duration of between 49 and 97 d. Infestations were higher in the middle and upper part of trees. Pinus kesiya and P. maximinoi had the highest levels of susceptibility in field as well as in greenhouse trials. A survey of naturally infested trees showed P. tecunumanii to be moderately susceptible, whereas P. patula and P. oocarpa had low levels of susceptibility in a greenhouse trial but were not susceptible in the field. Investigations considering the impact of predation of Ceraeochrysa species showed a high predation rate of up to 140 P. boerneri consumed per day by a single Ceraeochrysa individual. Other predators of P. boerneri were recorded but were not sufficiently common to warrant detailed study. 相似文献
73.
Vaidya NA Mathias K Ismail B Hayes KD Corvalan CM 《Journal of agricultural and food chemistry》2007,55(9):3408-3413
The conversion and degradation of malonylglucosides were kinetically characterized under elevated pH/heat conditions. Malonylgenistin and malonyldaidzin were heated at 60, 80, and 100 degrees C and pH values of 8.5, 9, and 9.5. A simple kinetic model was developed, which adequately predicted the conversion and degradation reactions. The conversion and degradation rates increased as temperature and pH increased. The rates of conversion of both malonylglucosides into their respective beta-glucosides were comparable under all pH/heat treatments. However, at 100 degrees C, the rates of degradation of malonyldaidzin were approximately double those of malonylgenistin, under all pH treatments. When malonlydaidzin was heated at 100 degrees C and pH 9.5, degradation of the produced daidzin occurred. Therefore, an alternative kinetic model was developed to better predict the conversion and degradation of malonyldaidzin occurring at 100 degrees C and pH 9.5. The models developed provide soy food manufacturers with guidelines for better control of the profile and level of isoflavones.. 相似文献
74.
75.
Manganaris GA Vicente AR Crisosto CH Labavitch JM 《Journal of agricultural and food chemistry》2007,55(17):7015-7020
The effect of postharvest dips in a 1-methylcyclopropene-generating solution of the formulation AFxRD-038 (Rohm & Haas) on plum fruit (Prunus salicina Lindell cv. 'Harrow Sun') quality and ripening during storage was determined. Fruit weight loss, tissue firmness, soluble solids content (SSC), titratable acidity (TA), ethylene production, respiration, and the activities of the cell wall modifying enzymes polygalacturonase (PG), 1,4-beta-D-glucanase/glucosidase (EGase), beta-galactosidase (beta-gal), and pectin methylesterase (PME) were measured. Fruit reddening, anthocyanin content, and phenylalanine ammonia-lyase (PAL) activity were also analyzed. The 1-MCP-treated fruit showed reduced ethylene production and respiration rate and delayed softening, which was associated with the reduction in the activity of PG, EGase, and beta-gal. The immersion in 1-MCP-generating solutions also decreased weight and acidity loss without modifying the fruit SSC. The immersion treatment was particularly effective in the fruit stored at 5 degrees C, keeping higher overall quality, maintaining lower levels of anthocyanins and PAL activity, and preventing flesh reddening. The present data show that beneficial effects in delaying plum fruit ripening and controlling chilling injury can be obtained by dipping the fruits in a solution of this novel 1-MCP-generating formulation. 相似文献
76.
Duarte-Vázquez MA García-Padilla S García-Almendárez BE Whitaker JR Regalado C 《Journal of agricultural and food chemistry》2007,55(25):10396-10404
A peroxidase isozyme (BP) was purified to homogeneity from broccoli stems ( Brassica oleraceae var. maraton) discarded from industrial processing wastes. BP specific activity was 1216 ABTS [2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)] units/mg, representing 466-fold that of crude extract. BP is a monomeric glycoprotein containing 16% carbohydrates, with a molecular mass of 49 kDa and an isoelectric point close to 4.2. From kinetic data it showed a two-substrate ping-pong mechanism, and the catalytic efficiency measured as the rate-limiting step of free BP regeneration was 3.4 x 10(6) M(-1) s(-1). The ABTS K m value was 0.2 mM, which was about 20 times lower than that reported for acidic commercial horseradish peroxidase (HRP). Assessment of BP secondary structure showed 30% helical character, similar to HRP and cytochrome c peroxidase. BP lost only 25% activity after 10 min of heating at 55 degrees C and pH 6; it was stable in the pH range from 4 to 9 and showed an optimum pH of 4.6 using ABTS as substrate. BP was active on substrates normally involved in lignin biosynthesis, such as caffeic and ferulic acids, and also displayed good catechol oxidation activity in the presence of hydrogen peroxide. Reverse micellar extraction was successfully used as potential large-scale prepurification of broccoli peroxidase, achieving a purification factor of 7, with 60% activity yield. Stems from the broccoli processing industry have a high potential as an alternative for peroxidase purification. 相似文献
77.
Carlos O. Schild Fabiana M. Boabaid Luiz G.S. Olivera Mizael Machado Ana Vildoza Anderson Saravia Alejandra Custodio Carolina Command Agustín Martinez Martín Jaurena Rob Dixon Franklin Riet-Correa 《Journal of veterinary diagnostic investigation》2021,33(5):1018
We investigated 2 outbreaks of osteomalacia as a result of phosphorus (P) deficiency in herds of lactating beef cows grazing subtropical native pastures in Uruguay. Cows exhibited pica, difficulty to stand and walk, rib fractures, and body weight loss even with adequate forage availability. Osteopenia and severe osteomalacia were observed on gross and histologic examination. The concentrations of bicarbonate-extractable P in soil (4.0, 4.1 mg P/kg), total P in pasture (0.9, 1.1 g P/kg), inorganic P in serum (1.0, 0.71 mmol P/L), and P in bone (73 mg P/mL) were all low. Although injectable and mineral salt supplements provided additional P in both outbreaks, these supplementary amounts were insufficient to prevent P deficiency. The P ingested by the cows from the pasture and supplements would have provided 20–55% of their daily P requirements of ~21 g P/d. Osteomalacia occurred in cattle at the 2 ranches as a result of severe P deficiency in the soil and forage, and inadequate P supplementation. Following diagnosis, control of P deficiency in beef cattle requires estimation of the amount of pasture P ingested and provision of sufficient additional supplementary P to meet the animals’ requirements. 相似文献
78.
Neira Pablo Henríquez-Castillo Carlos Ortiz Juan Stolpe Neal Dube Francis 《Agroforestry Systems》2021,95(8):1465-1478
Agroforestry Systems - Soil biota is considered a crucial component of soil health. Currently, the soil microbiota and its genomic material (microbiome) have received increasing attention due to... 相似文献
79.
Tomato mottle mosaic virus: Characterization,resistance gene effectiveness,and quintuplex RT-PCR detection system 下载免费PDF全文
Carlos Kwesi TETTEY YAN Zhi-yong MA Hua-yu ZHAO Mei-sheng GENG Chao TIAN Yan-ping LI Xiang-dong 《农业科学学报》2022,21(9):2641-2651
Tomato mottle mosaic virus (ToMMV), an economically important species of the genus Tobamovirus, causes significant loss in yield and quality of tomato fruits. Here, we identified the Shandong isolate of ToMMV (ToMMV-SD) collected from symptomatic tomato fruits in Weifang, Shandong Province of China. ToMMV-SD caused symptoms such as severe mosaic, mottling, and necrosis of tomato leaves, yellow spot and necrotic lesions on tomato fruits. The obtained full genome of ToMMV-SD was 6 399 nucleotides (accession number MW373515) and had the highest identity of 99.5% with that of isolate SC13-051 from the United States of America at the genomic level. The infectious clone of ToMMV-SD was constructed and induced clear mosaic and necrotic symptoms onto Nicotiana benthamiana leaves. Several commercial tomato cultivars, harboring Tm-22 resistance gene, and pepper cultivars, containing L resistance gene, were susceptible to ToMMV-SD. Plants of Solanum melongena (eggplant) and Brassica pekinensis (napa cabbage) showed mottling symptoms, while N. tabacum cv. Zhongyan 100 displayed latent infection. ToMMV-SD did not infect plants of N. tabacum cv. Xanthi NN, Brassica rapa ssp. chinensis (bok choy), Raphanus sativus (radish), Vigna unguiculata cv. Yuanzhong 28-2 (cowpea), or Tm-22 transgenic N. benthamiana. A quintuplex RT-PCR system differentiated ToMMV from tomato mosaic virus, tomato brown rugose fruit virus, tobacco mosaic virus, and tomato spotted wilt virus, with the threshold amount of 0.02 pg. These results highlight the threat posed by ToMMV to tomato and pepper cultivation and offer an efficient detection system for the simultaneous detection of four tobamoviruses and tomato spotted wilt virus infecting tomato plants in the field. 相似文献
80.
Bentonite-humic acid improves soil organic carbon,microbial biomass,enzyme activities and grain quality in a sandy soil cropped to maize (Zea mays L.) in a semi-arid region 下载免费PDF全文
ZHOU Lei XU Sheng-tao Carlos M. MONREAL Neil B. MCLAUGHLIN ZHAO Bao-ping LIU Jing-hui HAO Guo-cheng 《农业科学学报》2022,21(1):208-221
A bentonite-humic acid (B-HA) mixture added to degraded soils may improve soil physical and hydraulic properties, due to effects such as improved soil structure and increased water and nutrient retention, but its effect on soil physicochemical and biological properties, and grain quality is largely unknown. The effect of B-HA, added at 30 Mg ha−1, was studied at 1, 3, 5 and 7 years after its addition to a degraded sandy soil in a semi-arid region of China. The addition of B-HA significantly increased water-filled pore space and soil organic carbon, especially at 3 to 5 years after its soil addition to the soil. Amending the sandy soil with B-HA also increased the content of microbial biomass (MB)-carbon, -nitrogen and -phosphorus, and the activities of urease, invertase, catalase and alkaline phosphatase. The significant effect of maize (Zea mays L.) growth stage on soil MB and enzyme activities accounted for 58 and 84% of their total variation, respectively. In comparison, B-HA accounted for 8% of the total variability for each of the same two variables. B-HA significantly enhanced soil properties and the uptake of N and P by maize in semi-arid areas. The use of B-HA product would be an effective management strategy to reclaim degraded sandy soils and foster sustainable agriculture production in northeast China and regions of the world with similar soils and climate. 相似文献